Tuesday, July 10, 2007

Today, we met with Dr. Wachter who re-introduced me to PCR analysis. I was very excited to be performing this relatively simple procedure since I have been teaching it for many years but have never had the opportunity to actually perform such procedure. It was just as simple as I thought, except for the minute quantities we were dealing with. Apparently, many of the materials were simply purchased from larger lab corporations. Then, we had the opportunity to see how agarose gel was mixed. I have to admit that I felt much more comfortable in this type of lab. Later, we changed concentrations several times to get the correct amount of crystals for seeding for PS I. Then, we had to work with a dialysis tube and capillary with a very small "loop" and we had to place it in a miniscule tube without touching the side in a cold room at 4 degrees Celsius! Fun, adventurous activity!

1 comment:

Anonymous said...

Neat that you've talked about it so many times and now have had a chance to do it!