Today, we tried a dialysis technique to crystallize PS I which required the use of many different equations as well as a series of rather complicated steps such as preparation of probes,detremination of chrolophyll concentration, reactor assembly. We were able to see "fast and immediate crystallization," use the spectrometer again, and deliberately denature the protein. We learned the secret of crystallization of PSI- sometimes you need to use a lower salt concentration. We are "recrystallizing" essentially for PS I or will be microseeding tomorrow and working with Dr. Wachter for PCR.
This work was painstaking and complex to me. I am excited to see what will happen due to our groups' work.
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