Two labs were going on in parallel this morning. Di worked with Jennifer and myself in creating GFP. Di had put modified e-coli bacteria in a test tube to be multiplied. Then we added it to a flask with a liter of broth. Every hour we checked the bacteria growth by seeing the 600nm absorbtion of a sample. The bacteia had been modified with a plasmid which contained DNA for GFP and an antibiotic resistance protein. The antibiotic resistance was used because an antibiotic was added to the broth to kill any bacteria that was not resistant. After 3 hours there was enough bacteria. GTDP was added, and the temperature reduced. This stopped the bacteria multipling and instead in started to produce GFP.

Meanwhile prof Fromme was running a inorganic crystal lab using copper sulphate.