Two new crystallation techniques used. Looked at batch crstallation - saw crystals midway. Precipitate- crystals- nothing. Free interphase crystallization with twice the amount of salt and protein concentration but no mixing to thuis create a distinct crystal curve.
Vapor diffusion technique used which utilizes the equilibration of 2 solutions through air. More equations used- basic algebraic principles but we went through actual equations for understanding. Looked at other students' crystals- saw the clover shape of HIV crystals- very exciting. Spoke to Professor regarding HIV vaccine development and the difficulty in creating patterns for crystals without sharp edges.
Tomorrow- Look at crystals- meet with grad students proposals. Lots of hands-on- very pleased.
Thursday, June 28, 2007
6/27/07
After the creation of stock solutions, we were able to use such solutions to batch crystallize Lysozyme for crystallation. More formulas that we have used in the class but are now applying allowed us to get the basic concentrations. Use of automatic pippetters was a bit challenging and we had three trys to get it right. We learned from our mistakes much like our students would. Went to the Biodesign Institute for a tour on the truly interdisciplinary research that is occuring there. Asked about prion research which they may be performing there- interested in personally finding out more.
Tuesday, June 26, 2007
6/26/07
- Applying molarity to lab situation
- Creating buffer solutions
- Titrations
- PowerPoint as to PS I and PS II
- Vocabulary- carotenoids, chlrophyll, quinones, trimers, thylakoid membrane, cyanobacteria- became cholorplasts- "slaves" of plant cells, oxygen toxicity i.e. combustion, reactions, contribute more oxygen to the atmosphere than the tropical rainforests, ATP synthase, splitting of water, reduction of hydrogen into NADPH
- Crystallation of proteins- best in zero gravity- want at atomic level
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